Visualization of New Ultrastructural Components in the Cell Wall of Candida Albicans with Fixatives Containing Tapo
نویسندگان
چکیده
INTRODUCTION Electron microscope studies of yeasts have revealed the laminated structure of the cell wall (1, 9, 10, 19), its major chemical constituents being distributed in separate layers (13, 15) . The number of layers and their morphology are, however, a matter of controversy mainly because the methods of chemical fixation of yeast cells for electron microscopy employed to date have been inadequate (11, 16) . In the search for an effective fixative for the
منابع مشابه
The Effects of Candida Albicans Cell Wall Protein Fraction on Dendritic Cell Maturation
Back ground: Candida albicans is a member of the normal human microflora. C. albicans cell wall is composed of several protein and carbohydrate components which have been shown to play a crucial role in C. albicans interaction with the host immune system. Major components of C. albican cell wall are carbohydrates such as mannans, β glucans and chitins, and proteins that partially modulate the h...
متن کاملThe Preservation of Subcellular Organelles of Candida Albicans with Conventional Fixatives
In an approach to a study of the action of fungicidal drugs on the morphology of Candida albicans, we were faced with the problem of the preservation of substructures in untreated control cells . In contrast to results obtained with other yeast cells such as Cryptococcus neoformans (2, 9), chemical fixation with OsO, or with glutaraldehyde proved to be unsatisfactory for the preservation of the...
متن کاملComparison of cell wall proteins in putative Candida albicans & Candida dubliniensis by using modified staining method & SDSPAGE
Background: Candida species are among the most common causes of opportunistic fungal diseases. Among Candida species, Candida albicans is responsible for most infections. Having many strains, C. albicans is very polymorph. C. dubliniensis is very similar to albicans species both morphologically and physiologically. For an infection to occur, cell wall proteins play an important role as they en...
متن کاملEffect of Cell Wall, Cytoplasmic Fraction and Killed-Candida albicans on Nitric Oxide Production by Peritoneal Macrophages from BALB/c Mice
Objective(s) The fractions of Candida albicans have been used as an immunomodulator. The present work assessed the effect of different fractions of C. albicans on nitric oxide (NO) production by mice peritoneal macrophages. Materials and Methods Cell wall and cytoplasmic fractions of C. albicans ATCC 10321 strain were extracted. Mice peritoneal macrophages were purified and cultured. Differen...
متن کاملHwp1 gene Expression of Candida albicans and Study its role in adherence
Candida albicans is an opportunistic fungal pathogen found as mycoflora in the human body surfaces. Sevral genes play a crucial rule in its virulence including Hwp1 (hyphal wall protein 1), BCR1 and ALS gene family. Hwp1 gene is a responsible for coding a cell surface protein, which mediates biofilm formation in candida albicans. Here we investigated the presence of the HWP1gene was characteris...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of Cell Biology
دوره 52 شماره
صفحات -
تاریخ انتشار 1972